Refined X-ray Structures of Haloalkane Dehalogenase at pH 6·2 and pH 8·2 and Implications for the Reaction Mechanism
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چکیده
منابع مشابه
A pH-indicator-based screen for hydrolytic haloalkane dehalogenase.
1. Introduction Microbial hydrolytic haloalkane dehalogenases catalyze the cleavage of halogen-carbon bonds of a variety of aliphatic halogenated compounds, including a broad range of chlorinated (C 2 –C 6) and brominated (C 2 –C 8) alkanes, with water as the sole co-substrate, resulting in the production of halide ions, protons, and alcohols (1,2). Based primarily on substrate specificity and ...
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Haloalkane dehalogenase (DhlA) catalyzes the hydrolysis of haloalkanes via an alkyl-enzyme intermediate. Trp175 forms a halogen/halide-binding site in the active-site cavity together with Trp125. To get more insight in the role of Trp175 in DhlA, we mutated residue 175 and explored the kinetics and X-ray structure of the Trp175Tyr enzyme. The mutagenesis study indicated that an aromatic residue...
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Capturing transient structures in the elimination reaction of haloalkane in solution by transient X-ray diffraction.
This Communication reports simultaneous tracking of structural and kinetic information for the photoinduced elimination reaction of 1,2-diiodotetrafluoroethane in solution by transient X-ray diffraction. The transient structure of .CF2CF2I is determined to be a classical mixture whereas .CH2CH2I is bridged. Compared with the gas phase reaction, the secondary dissociation of .CF2CF2I into C2F4 a...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 1993
ISSN: 0022-2836
DOI: 10.1006/jmbi.1993.1436